{"id":29397,"date":"2022-06-18T18:34:51","date_gmt":"2022-06-18T16:34:51","guid":{"rendered":"https:\/\/www2.zoolyx.be\/faq-items\/quest-ce-que-le-rt-qpcr-et-quelle-est-la-difference-avec-le-pcr-conventionnel\/"},"modified":"2022-06-18T18:34:51","modified_gmt":"2022-06-18T16:34:51","slug":"quest-ce-que-le-rt-qpcr-et-quelle-est-la-difference-avec-le-pcr-conventionnel","status":"publish","type":"avada_faq","link":"https:\/\/www2.zoolyx.be\/fr\/faq-items\/quest-ce-que-le-rt-qpcr-et-quelle-est-la-difference-avec-le-pcr-conventionnel\/","title":{"rendered":"Qu\u2019est-ce que le RT-qPCR et quelle est la diff\u00e9rence avec le PCR conventionnel?"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"max-width:1289.6px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-1\" style=\"--awb-text-transform:none;\"><p>Les deux techniques utilisent des <strong>primers<\/strong> qui d\u00e9tectent un morceau de code g\u00e9n\u00e9tique caract\u00e9ristique de ce que l\u2019on recherche dans l&rsquo;\u00e9chantillon. La diff\u00e9rence avec RT-qPCR est l\u2019utilisation suppl\u00e9mentaire d\u2019<strong>une sonde (probe) fluorescente.<\/strong> Cette sonde, \u00e9galement caract\u00e9ristique pour le code recherch\u00e9, se lie un peu loin des primers.<\/p>\n<p>La pi\u00e8ce manquante entre les primers et la sonde est compl\u00e9t\u00e9e par <strong>la polym\u00e9rase Taq<\/strong>. Au moment o\u00f9 la cha\u00eene est enti\u00e8rement copi\u00e9e, la sonde lib\u00e8re son label fluorescente.<\/p>\n<p>\u00c0 la fin du cycle, une copie est produite qui est utilis\u00e9e ensemble avec l\u2019original dans le cycle suivant pour les copier \u00e0 nouveau. Comme le nombre de copies double au cours de cycles successifs, la fluorescence augmente de fa\u00e7on exponentielle.<\/p>\n<p>Cependant, \u00e0 mesure que les cycles progressent, les r\u00e9actifs sont \u00e9puis\u00e9s et constituent un facteur limitant pour l\u2019augmentation exponentielle, l\u2019augmentation de la fluorescence ralentit et cela de plus en plus rapide.<\/p>\n<p><strong>R&amp;ndy l\u2019explique aussi en d\u00e9tail dans ce film.<\/strong><\/p>\n<\/div><div class=\"fusion-video fusion-youtube\" style=\"--awb-max-width:600px;--awb-max-height:360px;--awb-align-self:center;--awb-width:100%;\"><div class=\"video-shortcode\"><div class=\"fluid-width-video-wrapper\" style=\"padding-top:60%;\" ><iframe title=\"YouTube video player 1\" src=\"https:\/\/www.youtube.com\/embed\/xa7izK2JiyM?wmode=transparent&autoplay=0&amp;rel=0&enablejsapi=1\" width=\"600\" height=\"360\" allowfullscreen allow=\"autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture;\"><\/iframe><\/div><\/div><\/div><div class=\"fusion-text fusion-text-2\"><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":3,"featured_media":0,"menu_order":14,"comment_status":"open","ping_status":"closed","template":"","format":"standard","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_price":"","_stock":"","_tribe_ticket_header":"","_tribe_default_ticket_provider":"","_tribe_ticket_capacity":"0","_ticket_start_date":"","_ticket_end_date":"","_tribe_ticket_show_description":"","_tribe_ticket_show_not_going":false,"_tribe_ticket_use_global_stock":"","_tribe_ticket_global_stock_level":"","_global_stock_mode":"","_global_stock_cap":"","_tribe_rsvp_for_event":"","_tribe_ticket_going_count":"","_tribe_ticket_not_going_count":"","_tribe_tickets_list":"[]","_tribe_ticket_has_attendee_info_fields":false,"footnotes":""},"faq_category":[523],"class_list":["post-29397","avada_faq","type-avada_faq","status-publish","format-standard","hentry","faq_category-pcr-fr"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Qu\u2019est-ce que le RT-qPCR et quelle est la diff\u00e9rence avec le PCR conventionnel? 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