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ALT – Method

Test principle

ALT catalyzes the reaction between L-alanine and 2-oxoglutarate. The pyruvate formed is reduced by NADH in a reaction catalyzed by lactate dehydrogenase (LDH) to form L-lactate and NAD+. The rate of the NADH oxidation is directly proportional to the catalytic ALT activity. It is determined by measuring the decrease in absorbance.

L-alanine + α-ketoglutarate  > ALT pyruvate + L-glutamate

Pyruvate + NADH + H+ > LDH L-lactate + NAD+


Laatst geupdate op 27 december 2022 om 12:52

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